Compare Between Plasmid,BAC,Clonning Vector:

Plasmid: The size of Plasmid is 2.5-20kb.. It's very small piece of DNA often form in bacteria.

BAC: BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

Cloning Vector: Cloning Vector is a small piece of DNA, tsken form a virous, a plasmid, or the cell of a higer organism, that can be stably maintained in an organism.

Comparing between Plasmid, BAC and Cloning Vector:

1.Plasmid:Plasmids are the most-commonly used bacterial cloning vectors

2.BAC: A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid , used for transforming and cloning in bacteria.

3.Cloning: A cloning vector is a small piece of DNA that can be stably maintained in an organism.

Plasmid vectors  are small, double-stranded circular DNA molecules with a bacterial replication origin capable of producing high levels of replication (hundreds of copies can be made per cell) and convenient restriction sites.

A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division

A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium

Compare Between Plasmid,BAC,Clonning Vector:

Plasmid:A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it. They generally carry only a small number of genes, notably some associated with antibiotic resistance. Plasmids may be passed between different bacterial cells.

BAC:BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication 

Clonning Vector:A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Cloning Vector: Cloning Vector is a small piece of DNA, tsken form a virous, a plasmid, or the cell of a higer organism, that can be stably maintained in an organism.

Compare Between Plasmid,BAC,Clonning Vector:
Plasmid vectors :    plasmid vectors are small, double-stranded circular DNA molecules with a bacterial replication origin capable of producing high levels of replication (hundreds of copies can be made per cell) and convenient restriction sites.

BAC:   A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division

Cloning vector:   A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium

Plasmid: The size of Plasmid is 2.5-20kb.It's very small piece of DNA often form in bacteria. It is  the most common used of bacterial cloning vectors.

BAC[ Bacterial Artificial Chromosome]: BAC is a DNA construct, based on a functional fertility plasmid , used for transforming and cloning in bacteria. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

Cloning Vector: Cloning Vector is a small piece of DNA, a virous, a plasmid or the cell of a higer organism, that can be stably maintained in an organism.

Plasmid:

A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria; however, plasmids are sometimes present in archaea and eukaryotic organisms.The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.

BAC:

 BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

cloning vector:

A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

There are many types of cloning vectors, but the most commonly used ones are genetically engineered plasmids. Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages, cosmids, and bacterial artificial chromosomes .All cloning vectors have features that allow a gene to be conveniently inserted into the vector or removed from it. This may be a multiple cloning site or polylinker, which contains many unique restriction sites.

Compare Between Plasmid,BAC,Clonning Vector:
Plasmid vectors :    plasmid vectors are small, double-stranded circular DNA molecules with a bacterial replication origin capable of producing high levels of replication (hundreds of copies can be made per cell) and convenient restriction sites.

BAC:   A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division

Cloning vector:   A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium

Cloning vector is a small piece of stable DNA into which a foreign DNA fragment can be inserted for cloning purposes. There are many types of cloning vectors, the most commonly used is genetically engineered plasmids. Cloning is generally first performed by using Escherichia coli, and cloning vectors in E. coli consists of plasmids, bacteriophages, cosmids, and bacterial artificial chromosomes (BACs).A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.[1] The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium. The vector therefore contains features that allow for the convenient insertion or removal of a DNA fragment to or from the vector, for example by treating the vector and the foreign DNA with a restriction enzyme that cuts the DNA. DNA fragments thus generated contain either blunt ends or overhangs known as sticky ends, and vector DNA and foreign DNA with compatible ends can then be joined together by molecular ligation. After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.

There are many types of cloning vectors, but the most commonly used ones are genetically engineered plasmids. Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages (such as phage λ), cosmids, and bacterial artificial chromosomes (BACs). Some DNA, however, cannot be stably maintained in E. coli, for example very large DNA fragments, and other organisms such as yeast may be used. Cloning vectors in yeast include yeast artificial chromosomes (YACs).

Compare Between Plasmid,BAC,Clonning Vector:

Plasmid: The size of Plasmid is 2.5-20kb.. It's very small piece of DNA often form in bacteria.

BAC: BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

Cloning Vector: Cloning Vector is a small piece of DNA, tsken form a virous, a plasmid, or the cell of a higer organism, that can be stably maintained in an organism.

Compare Between Plasmid,BAC,Clonning Vector given below: 
Plasmid vectors :    plasmid vectors are small, double-stranded circular DNA molecules with a bacterial replication origin capable of producing high levels of replication (hundreds of copies can be made per cell) and convenient restriction sites.

BAC:   A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division

Cloning vector:   A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium

A cloning vector is a DNA molecule which can replicate in a suitable host organism, and into which a fragment of foreign DNA can be introduced. Most vectors used in molecular biology are based on bacterial plasmids and Bacteriophages (bacteria-infecting viruses). On the other hand expression vectors are  vector that, in addition to the properties of a cloning vector, contains sequences that direct expression of the inserted DNA sequence in an appropriate host organism. It is also known as an expression construct, a plasmid or virus designed for protein expression in cells.

(Plasmid):A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it. They generally carry only a small number of genes, notably some associated with antibiotic resistance. Plasmids may be passed between different bacterial cells.

(BAC):BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication 

(Clonning Vector):A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Plasmid: The size of Plasmid is 2.5-20kb.. It's very small piece of DNA often form in bacteria.

BAC: BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

Cloning Vector: Cloning Vector is a small piece of DNA, tsken form a virous, a plasmid, or the cell of a higer organism, that can be stably maintained in an organism.

Difference:

1.plasmid :Plasmids are the most-commonly used bacterial cloning vectors.The size of Plasmid is 2.5-20kb.. It's very small piece of DNA often form in bacteria.

2.BAC: A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid , used for transforming and cloning in bacteria.

3.Cloning: A cloning vector is a small piece of DNA that can be stably maintained in an organism.

A comparison between plasmid, BAC & cloning vector is given below:

Plasmid: A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it. They generally carry only a small number of genes, notably some associated with antibiotic resistance. Plasmids may be passed between different bacterial cells. 

Usually present in Bacterias like E-coli.

BAC: BAC means 'Bacterial Artificial Chromosome. It carries DNA from Humans or any other living being and is inserted into a host bacterium for replication.

Produced in labs and has more efficiency than a plasmid.

Cloning Vector: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA was taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Used for cloning.

Comparison between Plasmid, BAC and cloning vector is given bellow :

Plasmid : A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently.

They are most commonly found as small circular, double-stranded DNA molecules in bacteria; 

Plasmids are sometimes present in archaea and eukaryotic organisms.

BAC : A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli.

These are a circular, bacterial F element-based plasmid containing an average of about 180–200 kb of genomic DNA.

They enable stable cloning of large DNA fragments from single genomes or microbial assemblages.

Cloning Vector : A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes

Cloning vector are useful for generating many copies of a gene.

Used as the vehicle for transporting foreign genetic material into another cell.

Compare Between Plasmid,BAC,Clonning Vector:
Plasmid vectors :    plasmid vectors are small, double-stranded circular DNA molecules with a bacterial replication origin capable of producing high levels of replication (hundreds of copies can be made per cell) and convenient restriction sites.

BAC:   A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division

Cloning vector:   A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium

A cloning vector is a DNA molecule which can replicate in a suitable host organism, and into which a fragment of foreign DNA can be introduced. Most vectors used in molecular biology are based on bacterial plasmids and Bacteriophages (bacteria-infecting viruses). On the other hand expression vectors are  vector that, in addition to the properties of a cloning vector, contains sequences that direct expression of the inserted DNA sequence in an appropriate host organism. It is also known as an expression construct, a plasmid or virus designed for protein expression in cells. BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

Answer: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.The restriction sites may be further used for sub-cloning into another vector if necessary. In this Topo-cloning method a linearized vector is activated by attaching topoisomerase I to its ends, and this "TOPO-activated" vector may then accept a PCR product by ligating both the 5' ends of the PCR product, releasing the topoisomerase and forming a circular vector in the process. In this Topo-cloning method a linearized vector is activated by attaching topoisomerase I to its ends, and this "TOPO-activated" vector may then accept a PCR product by ligating both the 5' ends of the PCR product, releasing the topoisomerase and forming a circular vector in the process.The gene, once cloned into the cloning vector (called entry clone in this method), may be conveniently introduced into a variety of expression vectors by recombination.

Compare Between Plasmid,BAC,Clonning Vector:

Plasmid:A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it. They generally carry only a small number of genes, notably some associated with antibiotic resistance. Plasmids may be passed between different bacterial cells.

BAC:BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication 

Clonning Vector:A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Compare Between Plasmid, BAC, Cloning Vector :

plasmid: Plasmids are autonomously replicating circular extra-chromosomal DNA. They are the standard cloning vectors and the ones most commonly used. Most general plasmids may be used to clone DNA insert of up to 15 kb in size. 

BAC: A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells. BACs are often used in connection with DNA sequencing. Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs. The BACs, with their inserted DNA, are then taken up by bacterial cells. As the bacterial cells grow and divide, they amplify the BAC DNA, which can then be isolated and used in sequencing DNA.

Cloning : A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Compare Between Plasmid,BAC,Clonning Vector:

Plasmid:A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it. They generally carry only a small number of genes, notably some associated with antibiotic resistance. Plasmids may be passed between different bacterial cells.

BAC:BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication 

Clonning Vector:A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Answer: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.The restriction sites may be further used for sub-cloning into another vector if necessary. In this Topo-cloning method a linearized vector is activated by attaching topoisomerase I to its ends, and this "TOPO-activated" vector may then accept a PCR product by ligating both the 5' ends of the PCR product, releasing the topoisomerase and forming a circular vector in the process. In this Topo-cloning method a linearized vector is activated by attaching topoisomerase I to its ends, and this "TOPO-activated" vector may then accept a PCR product by ligating both the 5' ends of the PCR product, releasing the topoisomerase and forming a circular vector in the process.The gene, once cloned into the cloning vector (called entry clone in this method), may be conveniently introduced into a variety of expression vectors by recombination.

Plasmid:A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it. They generally carry only a small number of genes, notably some associated with antibiotic resistance. 

BAC: It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

Cloning Vector: Cloning Vector is a small piece of DNA, a virous, a plasmid or the cell of a higer organism, that can be stably maintained in an organism.

Plasmid vectors are small, double-stranded circular DNA molecules with a bacterial replication origin capable of producing high levels of replication (hundreds of copies can be made per cell) and convenient restriction sites.

BAC vectors (Bacterial Artificial Chromosome)  are plasmids constructed with the replication origin of E. coli F factor, and so can be maintained in a single copy per cell. These vectors can hold DNA fragments of up to 300 kb.

Cloning vectors are useful for generating many copies of your gene.It’s  usually contain features associated with the insertion or removal of DNA fragments.

Plasmid : A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently.

They are most commonly found as small circular, double-stranded DNA molecules in bacteria; 

Plasmids are sometimes present in archaea and eukaryotic organisms.

BAC : A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli.

These are a circular, bacterial F element-based plasmid containing an average of about 180–200 kb of genomic DNA.

They enable stable cloning of large DNA fragments from single genomes or microbial assemblages.

Cloning Vector : A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes

Cloning vector are useful for generating many copies of a gene.

Used as the vehicle for transporting foreign genetic material into another cell.

Compare Between PLASMID , BAC, Clonning Vecto

 Plasmid:

A  plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes. Often, the genes carried in plasmids provide bacteria with genetic advantages, such as antibiotic resistance.

BAC[ Bacterial Artificial Chromosome]: BAC is a DNA construct, based on a functional fertility plasmid , used for transforming and cloning in bacteria. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

Cloning vector

The cloning vector may be DNA taken from a virus, the  cell of a higher organism, or it may be the plasmid of a bacterium.

Compare Between Plasmid, BAC, Cloning Vector

Ans: 

Plasmid

 A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria.

BAC

BAC mean Bacterial Artificial Chromosome. It carries DNA from Humans or any other living being and is inserted into a host bacterium for replication.

Cloning Vector

A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA was taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Comparing between Plasmid, BAC and Cloning Vector:

1.Plasmid:Plasmids are the most-commonly used bacterial cloning vectors

2.BAC: A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid , used for transforming and cloning in bacteria.

3.Cloning: A cloning vector is a small piece of DNA that can be stably maintained in an organism.

Comparison

Plasmid: A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it. They generally carry only a small number of genes, notably some associated with antibiotic resistance. 

BAC: It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

Cloning Vector: Cloning Vector is a small piece of DNA, a virous, a plasmid or the cell of a higer organism, that can be stably maintained in an organism.

A genetic structure in a cell that can replicate independently of the chromosomes, typically a small circular DNA strand in the cytoplasm of a bacterium or protozoan. Plasmids are much used in the laboratory manipulation of genes.

A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli.These are a circular, bacterial F element-based plasmid containing an average of about 180–200 kb of genomic DNA.They enable stable cloning of large DNA fragments from single genomes or microbial assemblages.

A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Compare Between Plasmid, BAC, Clonning Vector

Plasmid: A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it. They generally carry only a small number of genes, notably some associated with antibiotic resistance. Plasmids may be passed between different bacterial cells. 

BAC: BAC means 'Bacterial Artificial Chromosome. It carries DNA from Humans or any other living being and is inserted into a host bacterium for replication.

Cloning Vector: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA was taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Plasmid : 

A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria. Plasmids are sometimes present in archaea and eukaryotic organisms.

BAC : 

A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli. These are a circular, bacterial F element-based plasmid containing an average of about 180–200 kb of genomic DNA. They enable stable cloning of large DNA fragments from single genomes or microbial assemblages.

Cloning Vector : 

A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. Cloning vector are useful for generating many copies of a gene.

Plasmid : A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently.

BAC : A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria.

Clonning Vector : A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes.^[1] The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium. The vector therefore contains features that allow for the convenient insertion or removal of a DNA fragment to or from the vector, for example by treating the vector and the foreign DNA with a restriction enzyme that cuts the DNA. DNA fragments thus generated contain either blunt ends or overhangs known as sticky ends, and vector DNA and foreign DNA with compatible ends can then be joined together by molecular ligation. After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.

There are many types of cloning vectors, but the most commonly used ones are genetically engineered plasmids. Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages (such as phage λ), cosmids, and bacterial artificial chromosomes (BACs). Some DNA, however, cannot be stably maintained in E. coli, for example very large DNA fragments, and other organisms such as yeast may be used. Cloning vectors in yeast include yeast artificial chromosomes

Comparison between Plasmid, BAC and cloning vector is given bellow :

Plasmid : A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently.

They are most commonly found as small circular, double-stranded DNA molecules in bacteria; 

Plasmids are sometimes present in archaea and eukaryotic organisms.

BAC : A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli.

These are a circular, bacterial F element-based plasmid containing an average of about 180–200 kb of genomic DNA.

They enable stable cloning of large DNA fragments from single genomes or microbial assemblages.

Cloning Vector : A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes

Cloning vector are useful for generating many copies of a gene.

Used as the vehicle for transporting foreign genetic material into another cell.

Plasmid : 

A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria. Plasmids are sometimes present in archaea and eukaryotic organisms.

BAC : 

A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli. These are a circular, bacterial F element-based plasmid containing an average of about 180–200 kb of genomic DNA. They enable stable cloning of large DNA fragments from single genomes or microbial assemblages.

Cloning Vector : 

A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. Cloning vector are useful for generating many copies of a gene.

Comparison between Plasmid, BAC, Cloning vector - 

Plasmid - A genetic structure in a cell that can replicate independently of the chromosomes, typically a small circular DNA strand in the cytoplasm of a bacterium or protozoan. Plasmids are much used in the laboratory manipulation of genes.

BAC - A bacterial artificial chromosome (BAC) is an engineered DNA molecule used to clone DNA sequences in bacterial cells (for example, E. coli). BACs are often used in connection with DNA sequencing. Segments of an organism's DNA, ranging from 100,000 to about 300,000 base pairs, can be inserted into BACs. The BACs, with their inserted DNA, are then taken up by bacterial cells. As the bacterial cells grow and divide, they amplify the BAC DNA, which can then be isolated and used in sequencing DNA.

Cloning Vector - A DNA molecule in which when a foreign DNA is integrated has the capability to replicate it within itself to give birth to numerous clones of recombinant DNA is known as a cloning vector. Vectors that are used for cloning are plasmids and phages.

Plasmid: A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it. They generally carry only a small number of genes, notably some associated with antibiotic resistance. Plasmids may be passed between different bacterial cells.

BAC: BAA bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division.

Cloning Vector: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA was taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Plasmid vectors :    plasmid vectors are small, double-stranded circular DNA molecules with a bacterial replication origin capable of producing high levels of replication (hundreds of copies can be made per cell) and convenient restriction sites.

BAC:   A bacterial artificial chromosome is a DNA construct, based on a functional fertility plasmid, used for transforming and cloning in bacteria, usually E. coli. F-plasmids play a crucial role because they contain partition genes that promote the even distribution of plasmids after bacterial cell division

Cloning vector:   A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium

Compare Between Plasmid,BAC,Clonning Vector:

Plasmid: The size of Plasmid is 2.5-20kb.. It's very small piece of DNA often form in bacteria.

BAC: BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

Cloning Vector: Cloning Vector is a small piece of DNA, tsken form a virous, a plasmid, or the cell of a higer organism, that can be stably maintained in an organism.

DNA sequencing technique used to determine the nucleotide sequence of DNA

Cloning vector is one which can used for cloning and sequencing the gene of interest where as expression vectors contains tightly regulated sequences like selectable marker, Ribosomal binding site, terminator sequence.

A cloning vector is a DNA molecule that can replicate in a suitable host organism, and into which a fragment of foreign DNA can be introduced. Most vectors used in molecular biology are based on bacterial plasmids and Bacteriophages (bacteria-infecting viruses). On the other hand, expression vectors are vector that, in addition to the properties of a cloning vector, contain sequences that direct expression of the inserted DNA sequence in an appropriate host organism. It is also known as an expression construct, a plasmid or virus designed for protein expression in cells. 

A cloning vector is a DNA molecule that can replicate in a suitable host organism, and into which a fragment of foreign DNA can be introduced. Most vectors used in molecular biology are based on bacterial plasmids and Bacteriophages (bacteria-infecting viruses). On the other hand, expression vectors are vector that, in addition to the properties of a cloning vector, contain sequences that direct expression of the inserted DNA sequence in an appropriate host organism. It is also known as an expression construct, a plasmid or virus designed for protein expression in cells. 

Comparing between Plasmid, BAC, Cloning Vector

Compare Between Plasmid,BAC,Clonning Vector:

Plasmid:A plasmid is a small, often circular DNA molecule found in bacteria and other cells. Plasmids are separate from the bacterial chromosome and replicate independently of it. They generally carry only a small number of genes, notably some associated with antibiotic resistance. Plasmids may be passed between different bacterial cells.

BAC:BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication 

Clonning Vector:A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.

Plasmid: The size of Plasmid is 2.5-20kb.. It's very small piece of DNA often form in bacteria.

BAC: BAC mean Bacterial Artificial Chromosome. It's carry DNA from Humans or any other living being, and is inserted into a host bacterium for replication.

Cloning Vector: Cloning Vector is a small piece of DNA, tsken form a virous, a plasmid, or the cell of a higer organism, that can be stably maintained in an organism.

Comparing between Plasmid, BAC and Cloning Vector:

1.Plasmid:Plasmids are the most-commonly used bacterial cloning vectors

2.BAC: A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid , used for transforming and cloning in bacteria.

3.Cloning: A cloning vector is a small piece of DNA that can be stably maintained in an organism.

Comparing between Plasmid, BAC and Cloning Vector:

1.Plasmid:Plasmids are the most-commonly used bacterial cloning vectors

2.BAC: A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid , used for transforming and cloning in bacteria.

3.Cloning: A cloning vector is a small piece of DNA that can be stably maintained in an organism.

A cloning vector is a DNA molecule which can replicate in a suitable host organism, and into which a fragment of foreign DNA can be introduced. Most vectors used in molecular biology are based on bacterial plasmids and Bacteriophages (bacteria-infecting viruses). On the other hand expression vectors are  vector that, in addition to the properties of a cloning vector, contains sequences that direct expression of the inserted DNA sequence in an appropriate host organism. It is also known as an expression construct, a plasmid or virus designed for protein expression in cells.