Topic outline

  • 🎆 Welcome to the course!!!!

    afmi

  • Experiment no-01

    • Experiment NO: 01 -Introduction of culture media used in microbiology File

      • Culture media is of fundamental importance for most microbiological tests: to obtain pure cultures, to grow and count microbial cells, and to cultivate and select microorganisms. Without high-quality media, the possibility of achieving accurate, reproducible, and repeatable microbiological test results is reduced. A microbiological culture medium is a substance that encourages the growth, support, and survival of microorganisms. Culture media contains nutrients, growth promoting factors, energy sources, buffer salts, minerals, metals, and gelling agents (for solid media). Culture media has been used by microbiologists since the nineteenth century. Even with the increased use of rapid methods the majority of techniques found in the pharmaceutical quality control laboratory require growth media . For the assessment of culture media, no one definitive standard exists. In light of this, this article presents some considerations for designing the testing regime and for the selection and control of microorganisms.

  • Experiment Name: Catalase Test

    The catalase test is a biochemical test for aerobic organisms that detects the production of catalase enzyme in the organism.

    • Catalase enzyme is a common enzyme that is found in all living beings that survive in oxygen and catalyzes the decomposition of hydrogen peroxide, releasing water and oxygen.
    • Catalase is an essential enzyme in pathogenic organisms as it protects the organism from oxidative damage from the reactive oxygen species.
    • The enzyme neutralizes the bactericidal effects of hydrogen peroxide, and its concentration in bacteria has been correlated with the pathogenicity of the organism.
    • The catalase test has been extensively used over the years as it allows the differentiation of catalase-positive organisms like staphylococci from catalase-negative species like streptococci.
    • The catalase test is useful in the presumptive characterization of most bacteria.
    • Under the aerobic condition, 3% H2O2 is used, whereas 15% H2O2 is used under anaerobic conditions.

  • Experiment Name:03

    •  Selective media are used to select for the growth of a particular "selected" microorganism. For example, if a certain microbe is resistant to aparticular antibiotic (e.g., novobiocin), then that antibiotic can be added to the medium in order to prevent other organisms, which are not resistant, from growing. 
    • oBacterial Culture Media: Classification, Types, Uses • Microbe Online

  • Topic 4

    Microorganisms present in soil plays a major role in enhancing the plant growth. In our present study soil sample was collected from the agriculture field of Quantum Global Campus, Roorkee and bacterial organisms were isolated by serial dilution technique. Well defined isolated colonies were selected and pure cultured. The isolates were named as QAF01, QAF02, QAF03, QAF04 and QAF05. Biochemical characterization of the above mentioned isolates determined that 20% of the isolates were spore formers, 60% were motile in nature, 100% were rod shapers in morphology and 60% were gram positive in reaction. Metabolism of various sugars was done at a maximum rate by the isolates QAF04 and QAF03. Based on their biochemical characterization and carbohydrate fermentation the isolates were identified to be Bacillus, Pseudomonas, Streptomycis, Azotobacter and Alcaligenes.

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