Section outline

  • This chapter explores recombinant DNA technology, highlighting gene cloning, expression systems, and biotechnological production of pharmaceuticals, enzymes, and genetically modified organisms for medical, industrial, and agricultural applications.

    • resource icon
      BPH 421 L2.1 File
      Students must
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      CONTENTS

      • Basics of DNA
      • The Central Dogma
        Replication
        Transcription
        Translation
      • Recombinant DNA Technology
      • Preparation of Recombinant DNA

    • resource icon
      BPH 421 L2.1 Handnote File
      Students must
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      Do you want to know more about the central dogma of molecular biology?

      Then please go through this handnote which I have prepared for you! :)


    • resource icon
      BPH 421 L2.2 File
      Students must
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      CONTENTS

      • Preparation of Recombinant DNA
      • Enzymes & Restriction Enzymes
      • Naming Restriction Enzymes
      • Restriction-modification System
      • Recognition Site & Restriction Site
      • Vectors
      • Introducing Recombinant Vector

    • resource icon
      BPH 421 L2.2 Handnote File
      Students must
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      Why is it so important to have the ampR gene and lacZ gene in a plasmid?

      This handnote will try to answer all your questions which you had in the class! ;)

    • resource icon
      BPH 421 L2.3 File
      Students must
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      CONTENTS
      • DNA Amplification (PCR)
      • DNA Fractionation (Gel Electrophoresis)
      • DNA Analysis (Blotting)
      • DNA Fingerprinting

    • resource icon
      BPH 421 L 2.4 File